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IF mounting guide


Mounting Guide



Tips and tricks to improve your Immunofluorescence Results

Immunofluorescence (IF) is a powerful method for visualizing proteins expressed directly within tissues. Mounting, the last step in the IF workflow, is critical for tissue integrity, optical clarity, long-term storage, signal intensity, and fluorescence preservation. This IF mounting guide provides information to help researchers choose the best mounting medium for their project and offers tips to help reduce photobleaching during the image visualization process.


This guide includes:

  • An overview on fluorescence and photobleaching
  • Selection tips for using the appropriate mounting medium
  • A comparison of non-hardening vs hardening formulations
  • Other factors to consider when doing IF